ELISA Recombinant Novel Coronavirus Spike glycoprotein(S)(V367F),partial (Active)
Quantity:100µg.
Research Areas:Microbiology
Uniprot NO.:P0DTC2
Uniprot Entry Name:
Gene Names:S
Species:Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Source:Mammalian cell
Expression Region:319-541aa (V367F)
Sequence:RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSFLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Protein Description:Partial
Tag Info:C-terminal 10xHis-tagged
Mol. Weight:27.9 kDa
Biological_Activity:Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V367F) at 5 ?g/mL can bind ACE2 (CSB-MP866317HU), the EC50 is 65.58-90.16 ng/mL.
Purity:Greater than 90% as determined by SDS-PAGE.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
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Internal Reference:
CSB-MP3324GMY1(M1)