ELISA Recombinant N-terminal Xaa-Pro-Lys N-methyltransferase 1(NTMT1) (Active)

Quantity:1mg. Research Areas:Cell Biology Uniprot NO.:Q9BV86 Uniprot Entry Name: Gene Names:NTMT1 Species:Homo sapiens () Source:E.coli Expression Region:2-223aa Sequence:TSEVIEDEKQFYSKAKTYWKQIPPTVDGmLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR Protein Description:FµLl Length of Mature Protein Tag Info:N-terminal GST-tagged Mol. Weight:52.3 kDa Biological_Activity:Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 ?g/mL can bind NTMT1, the EC50 of NTMT1 protein is 3.029-5.404 ?g/mL. Purity:Greater than 85% as determined by SDS-PAGE. Endotoxin:Not test. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Alpha N-terminal protein methyltransferase 1AMethyltransferase-like protein 11AN-terminal R;CC1 methyltransferaseX-Pro-Lys N-terminal protein methyltransferase 1A ;NTM1A Relevance:Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. PubMed ID: Function: Involvement in disease: SubcellµLar Location: Protein Families: Tissue Specificity: Paythway: HGNC Database Link: UniGene Database Link: KEGG Database Link: STRING Database Link: OMIM Database Link: