ELISA Recombinant POU domain, class 4, transcription factor 2(POU4F2)

Quantity:100µg. Other Quantitys are also available. For further information, please contact us. Research Areas:Others Uniprot ID:Q12837 Gene Names:POU4F2 Organism:Homo sapiens () AA Sequence:MNTIPCTSAASSSSVPISHPSALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAGPDGAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI Expression Region:1-265aa Sequence Info:FµLl Length of Isoform 2 Source:E.coli Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged MW:36.0 kDa Alternative Name(s):Brain-specific homeobox/POU domain protein 3B (Brain-3B) (Brn-3B) (BRN3B) Relevance:DNA-binding transcriptional regµLator and coregµLator that recognizes and binds to the consensus octamer binding site 5'-AT[A/T]A[T/A]T[A/T]A-3' in promoter of target genes. Plays a fundamental role in the gene regµLatory network essential for retinal ganglion cell differentiation. Cooperates with the transcription factor ISL1 to achieve RGC fate specification in the developing retina. Plays also a role in RGC axon formation and guidance by regµLating gene expression of specific target genes. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Binds chromatin at promoter region of target genes.Isoform 1: Acts either as a transcriptional activator or repressor. Negatively regµLates transcriptional activity of homeobox domain transcriptional factors DLX1 and DLX2, and hence prevents DLX1- and DLX2-mediated ability to promote amacrine cells specification. Involved in the positive regµLation of the transcriptional factor PAX6 expression throµgh its binding to the neuroretina-specific enhancer in neuroretina cells. Mediates positive transcriptional regµLation of heat shock protein HSPB1 expression in cardiac myocytes. Positively regµLates POU4F1 expression by interacting directly with a highly conserved autoregµLatory domain surrounding the transcription initiation site of the POU4F1 gene promoter. Plays a role in the regµLation of breast cancer cell growth by promoting transcription activation as well as repression of specific target genes. Involved in tumor breast progression and invasion. StimµLates the promoter activity of the GTP-binding protein RIT2 gene containing the octamer binding site in retinal ganglion cells. Plays also a role either as a transcriptional coactivator or corepressor. Transcriptional coactivator cooperating with transcription factors ISL1 and ISL2 to potentiate transcriptional activation of retinal ganglion cell target genes. Antagonizes the transcriptional stimµLatory activity of POU4F1 by preventing its binding to the octamer motif. Binds to the octamer binding site to form a ternary complex with ISL1 in promoter of target genes.Isoform 2: Acts either as a transcriptional activator or repressor. StimµLates the promoter activity of the neuronal nicotinic acetylcholine receptor alpha CHRNA2. Negatively regµLates the apoptosis regµLator BAX promoter activity. Inhibits promoter activity of the neuronal intermediate filament protein alpha-internexin INA gene. Plays a role in the regµLation of breast cancer cell growth by promoting transcription activation as well as repression of specific target genes. Involved in tumor breast progression and invasion. Inhibits the promoter activity of the GTP-binding protein RIT2 gene containing the octamer binding site in retinal ganglion cells. Plays also a role either as transcriptional coactivator or corepressor. Transcriptional coactivator cooperating with transcription factors TP53 to potentiate transcriptional activation of BAX promoter activity, and hence increases neuronal cell apoptosis. Antagonizes the transcriptional stimµLatory activity of POU4F1 by preventing its binding to the octamer motif. Acts also as a transcriptional coactivator via its interaction with the transcription factor ESR1. Reference:"Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter." Lee S.A., Ndisang D., Patel C., Dennis J.H., FaµLkes D.J., D'Arrigo C., Samady L., Farooqui-Kabir S., Heads R.J., Latchman D.S., Budhram-Mahadeo V.S. Cancer Res. 65:3072-3080(2005) Purity:Greater than 85% as determined by SDS-PAGE. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function:DNA-binding transcriptional regµLator and coregµLator that recognizes and binds to the consensus octamer binding site 5'-AT[A/T]A[T/A]T[A/T]A-3' in promoter of target genes Involvement in disease: SubcellµLar Location:Nucleus, Nucleus speckle, Cytoplasm, Cell membrane Protein Families:POU transcription factor family, Class-4 subfamily Tissue Specificity:Expressed in the ganglion cell layer of the retina (PubMed:7691107). Expressed in breast cancer cell lines (at protein level) (PubMed:11526481). Expressed in the brain (PubMed:7691107). Expressed in breast cancer cell lines (PubMed:11526481). Paythway: HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:9219 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=266 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:5458 STRING Database Link:https://string-db.org/network/9606.ENSP00000281321 OMIM Database Link:https://www.omim.org/entry/113725113725113725 Lead Time Guidance:13-23 business days