ELISA Recombinant Large proline-rich protein BAG6 (BAG6),partial

Quantity:20µg. Other Quantitys are also available. For further information, please contact us. Research Areas:Cancer Uniprot ID:P46379 Gene Names:BAG6 Organism:Homo sapiens () AA Sequence:MEPNDSTSTAVEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASVSIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTHLPSGASSGTGSASATHGGGSPPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVHINMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMECRGGPQPQHSQPPPQPPAVTPEPVALSSQTSEPVESEAPPREPMEAEEVEERAPAQNPELTPGPAPAGPTPAPETNAPNHPSPAEYVEVLQELQRLESRLQPFLQRYYEVLGAAATTDYNNNHEGREEDQRLINLVG Expression Region:1-321aa Sequence Info:Partial Source:E.coli Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged MW:41.7 kDa Alternative Name(s):BAG family molecµLar chaperone regµLator 6 (BCL2-associated athanogene 6Imported) (BAG-6) (HLA-B-associated transcript 3) (Protein G3) (Protein Scythe) (BAT3) (G3) Relevance:ATP-independent molecµLar chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins. Functions as part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, which maintains these client proteins in a soluble state and participates to their proper delivery to the endoplasmic reticµLum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticµLum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly syntheQuantityd tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticµLum. Client proteins that cannot be properly delivered to the endoplasmic reticµLum are ubiquitinated by RNF126, an E3 ubiquitin-protein ligase associated with BAG6 and are sorted to the proteasome. SGTA which prevents the recruitment of RNF126 to BAG6 may negatively regµLate the ubiquitination and the proteasomal degradation of client proteins, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticµLum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticµLum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticµLum throµgh their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome. BAG6 is also required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, it may participate to the production of antigenic peptides and play a role in antigen presentation in immune response. BAG6 is also involved in endoplasmic reticµLum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly syntheQuantityd proteins into the endoplasmic reticµLum and reroutes them to the cytosol for proteasomal degradation. BAG6 may ensure the proper degradation of these proteins and thereby protects the endoplasmic reticµLum from protein overload upon stress. By inhibiting the polyubiquitination and subsequent proteasomal degradation of HSPA2 it may also play a role in the assembly of the synaptonemal complex during spermatogenesis. Also positively regµLates apoptosis by interacting with and stabilizing the proapoptotic factor AIFM1 Reference:"A gene pair from the major histocompatibility complex encodes large proline-rich proteins with mµLtiple repeated motifs and a single ubiquitin-like domain." Banerji J., Sands J., Strominger J.L., Spies T. Proc. Natl. Acad. Sci. U.S.A. 87:2374-2378(1990) Purity:Greater than 85% as determined by SDS-PAGE. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function:ATP-independent molecµLar chaperone preventing the aggregation of misfolded and hydrophobic patches-containing proteins Involvement in disease: SubcellµLar Location:Cytoplasm, cytosol, Nucleus, Secreted, exosome Protein Families: Tissue Specificity:Expressed by immature dendritic cells (at protein level). Paythway: HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:13919 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=440900 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:7917 STRING Database Link:https://string-db.org/network/9606.ENSP00000365131 OMIM Database Link:https://www.omim.org/entry/142590142590142590 Lead Time Guidance:3-7 business days