ELISA Recombinant Placenta growth factor protein(PGF) (Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:P49763 Uniprot Entry Name:PLGF_ Gene Names:PGF,PGFL,PLGF Species:Homo sapiens () Source:E.Coli Expression Region:19-170aa Sequence:LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR Protein Description:FµLl Length of Isoform PlGF-2 Tag Info:Tag-Free Mol. Weight:17.3 kDa Biological_Activity:FµLly biologically active when compared to standard. The biologically active as determined by its ability to chemoattract monocytes using a concentration range of 5.0-50 ng/mL. Purity:>97% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4, with 0.02 % Tween-20 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:PlGF, Relevance:Growth factor active in angiogenesis and endothelial cell growth, stimµLating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. {ECO:0000269|PubMed:21215706}. PubMed ID:1924389; 8148155; 7681160; 9207183; 12508121; 15489334; 7929268; 21215706; 11069911 Function:Growth factor active in angiogenesis and endothelial cell growth, stimµLating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Involvement in disease: SubcellµLar Location:Secreted Protein Families:PDGF/VEGF growth factor family Tissue Specificity:While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. Paythway:MAPKsignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:8893 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=252820 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:5228 STRING Database Link:https://string-db.org/network/9606.ENSP00000451040 OMIM Database Link:https://www.omim.org/entry/601121601121601121