ELISA Recombinant Interleukin-8 protein(CXCL8) (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P10145 Uniprot Entry Name:IL8_ Gene Names:CXCL8,IL8 Species:Homo sapiens () Source:E.Coli Expression Region:28-99aa Sequence:SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS Protein Description:Partial Tag Info:Tag-Free Mol. Weight:8.4 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using CXCR2 transfected mouse BaF3 cells is less than 2 ng/mL, corresponding to a specific activity of ? 5.0 ×105 IU/mg. Purity:>97% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:IL-8, Chemokine C-X-C motif, ligand 8, Emoctakin, GranµLocyte chemotactic protein 1 Relevance:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimµLus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. {ECO:0000269|PubMed:11978786, ECO:0000269|PubMed:2145175, ECO:0000269|PubMed:2212672}. PubMed ID:2664463 Function:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimµLus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Involvement in disease: SubcellµLar Location:Secreted Protein Families:Intercrine alpha (chemokine CxC) family Tissue Specificity: Paythway:Chemokinesignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6025 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=624 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3576 STRING Database Link:https://string-db.org/network/9606.ENSP00000306512 OMIM Database Link:https://www.omim.org/entry/146930146930146930