ELISA Recombinant Interleukin-6 protein(IL6) (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:P05231 Uniprot Entry Name:IL6_ Gene Names:IL6,IFNB2 Species:Homo sapiens () Source:E.Coli Expression Region:30-212aa Sequence:VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM Protein Description:FµLl Length of Mature Protein Tag Info:Tag-Free Mol. Weight:20.7 kDa Biological_Activity:Assay #1: FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Assay #2: FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/mL, corresponding to a specific activity of > 1.25 ×106 IU/mg. Purity:>96% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:IL-6, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor Relevance:Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insµLin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. PubMed ID:3491322; 3500852; 3538015; 3023045; 3320204; 2789513; 3758081; 3266463; 1291290; 15489334; 3279116; 2610854; 1883960; 7851440; 2472117; 2037043; 8555185; 9159484; 9118960; 9769329; 11001912; 11355017; 12768442; 26091039 Function:Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insµLin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. Involvement in disease:Rheumatoid arthritis systemic juvenile (RASJ) SubcellµLar Location:Secreted Protein Families:IL-6 superfamily Tissue Specificity: Paythway:HIF-1signalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6018 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=654458 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3569 STRING Database Link:https://string-db.org/network/9606.ENSP00000258743 OMIM Database Link:https://www.omim.org/entry/147620147620147620