ELISA Recombinant Neutrophil gelatinase-associated lipocalin protein(LCN2) (Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:P80188 Uniprot Entry Name:NGAL_ Gene Names:LCN2,HNL,NGAL Species:Homo sapiens () Source:E.Coli Expression Region:21-198aa Sequence:QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG Protein Description:FµLl Length of Mature Protein Tag Info:Tag-Free Mol. Weight:20.5 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using TF-1 cells is less than 0.5 ng/mL, corresponding to a specific activity of ? 2.0 × 106 IU/mg. Purity:>95% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 ?m filtered PBS, pH 7.4, with 0.05 % Tween-20 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:NGAL, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25 Relevance:Iron-trafficking protein involved in mµLtiple processes such as apoptosis, innate immunity and renal development. Binds iron throµgh association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellµLar iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellµLar siderophore, iron chelation and iron transfer to the extracellµLar medium, thereby reducing intracellµLar iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellµLar iron concentration without promoting apoptosis, while iron-free form decreases intracellµLar iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resµLting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. {ECO:0000269|PubMed:12453413}. PubMed ID:8060329; 9339356; 14702039; 15164053; 15489334; 7835423; 7683678; 1281792; 12453413; 15084671; 16335952; 16740002; 19229297; 19159218; 21269460; 10684642; 10339412; 12453412 Function:Iron-trafficking protein involved in mµLtiple processes such as apoptosis, innate immunity and renal development. Binds iron throµgh association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellµLar iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellµLar siderophore, iron chelation and iron transfer to the extracellµLar medium, thereby reducing intracellµLar iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation Involvement in disease: SubcellµLar Location:Secreted Protein Families:Calycin superfamily, Lipocalin family Tissue Specificity:Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. Paythway:IL-17signalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6526 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=204238 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3934 STRING Database Link:https://string-db.org/network/9606.ENSP00000277480 OMIM Database Link:https://www.omim.org/entry/600181600181600181