ELISA Disks large-associated protein 3 (DLGAP3)

Reactivity: (Homo sapiens) UniProt:O95886 Abbreviation:DLGAP3 Alternative Names:DAP3; SAPAP3; OTTHUMP00000004180|PSD-95/SAP90-binding protein 3|SAP90/PSD 95 associated protein 3|SAP90/PSD-95-associated protein 3|disks large-associated protein 3 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.5% Inter-AssayCV:?8.1% Recovery:0.84 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DLGAP3 in samples. An antibody specific for DLGAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DLGAP3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Using a segment of rat Fak (PTK2) to screen an adµLt brain cDNA library, Bongiorno-Borbone et al. (2005) cloned DLGAP3, which they called SAPAP3. The deduced protein contains 977 amino acids. By Northern blot analysis, Takeuchi et al. (1997) found brain-specific expression of rat Sapap3. The rat protein localized to dendrites and cell bodies and was enriched in synaptosomal membrane fractions.By mutation analysis, Bongiorno-Borbone et al. (2005) demonstrated that the N-terminal half of SAPAP3 interacted with a C-terminal segment of rat Fak. They also found that SAPAP3 interacted with Pyk2 (PTK2B) in in vitro pµLl-down assays. In rat forebrain lysates, part of Fak and Pyk2 had a similar subcellµLar distribution as Sapap3 and Sap90/Psd95, which supported their functional association. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).