ELISA Dipeptidyl peptidase 2 (DPP7)

Reactivity: (Homo sapiens) UniProt:Q9UHL4 Abbreviation:DPP7 Alternative Names:DPP2; DPPII; QPP; carboxytripeptidase|dipeptidyl aminopeptidase II|dipeptidyl arylamidase II|dipeptidyl peptidase 7|dipeptidyl-peptidase II|quiescent cell proline dipeptidase Application:ELISA Range:78.1-5000 pg/mL Sensitivity:36 pg/mL Intra-AssayCV:?4.5% Inter-AssayCV:?7.7% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DPP7 in samples. An antibody specific for DPP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DPP7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP7 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dipeptidyl-peptidase 2 is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained throµgh suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.predicted 492-amino acid protein has a calcµLated molecµLar mass of 54.3 kD and contains the consensus sequence for the active-site serine residue of serine-type proteases. It shares 42% amino acid identity with the carboxypeptidase PRCP, with higher identity at the putative active-site residues. Northern blot analysis detected a 1.7-kb transcript in PBMCs and Jurkat T cells. SDS-PAGE analysis showed expression of a 58-kD protein. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).