ELISA Dual oxidase 2 (DUOX2)

Reactivity: (Homo sapiens) UniProt:Q9NRD8 Abbreviation:DUOX2 Alternative Names:LNOX2; NOXEF2; P138-TOX; TDH6; THOX2; NADH/NADPH thyroid oxidase p138-tox|NADPH oxidase/peroxidase DUOX2|NADPH thyroid oxidase 2|dual oxidase-like domains 2|flavoprotein NADPH oxidase|nicotinamide a Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.069 ng/mL Intra-AssayCV:?4.2% Inter-AssayCV:?8.5% Recovery:0.95 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DUOX2 in samples. An antibody specific for DUOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DUOX2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dual oxidase is an enzyme that was first identified in the mammalian thyroid gland. In s, two isoforms are found; hDUOX1 and hDUOX2 (this enzyme). The protein location is not exclusive to thyroid tissue; hDUOX1 is prominent in airway epithelial cells and hDUOX2 in the salivary glands and gastrointestinal tract.It has been sµggested that DUOX2 is the isoform to generate H2O2 utilized by thyroid peroxidase (TPO) for the biosynthesis of thyroid hormones, supported by the discovery of congenital hypothyroidism resµLtant from an inactivating mutation in the DUOX2 gene. The family currently has seven members including NOX1, NOX2 (formerly known as gp91phox), NOX3, NOX4, NOX5, DUOX1 and DUOX2.This protein is known as a dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).