ELISA EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2)

Reactivity: (Homo sapiens) UniProt:O95967 Abbreviation:EFEMP2 Alternative Names:FBLN4; MBP1; UPH1; fibµLin 4|mutant p53 binding protein 1 Application:ELISA Range:3.12-200 ng/mL Sensitivity:1.32 ng/mL Intra-AssayCV:?3.9% Inter-AssayCV:?7.9% Recovery:0.84 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate EFEMP2 in samples. An antibody specific for EFEMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFEMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for EFEMP2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFEMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:A large number of extracellµLar matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagµLation, activation of complement and determination of cell fate during development. FBLN4 contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.EFEMP1 is likewise expressed in a wide range of adµLt and fetal tissues. In contrast to EFEMP1, however, EFEMP2 was not significantly overexpressed in senescent or quiescent fibroblasts, sµggesting a diversity of function within this EGF-like domain subfamily. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).