VIASURE SARS-CoV-2, Flu & RSV Real Time PCR Detection Kit

VIASURE SARS-CoV-2, Flu & RSV Real Time PCR Detection Kit is a test kit designed for the qualitative detection of RNA from the SARS-CoV-2, Influenza A/B (Flu A/B) and/or Human Respiratory Syncytial Virus A/B (RSV A/B) in respiratory specimens from individuals using real-time RT-PCR method . This test does not make distinction between Influenza or RSV A and B types. RNA is extracted from respiratory specimens, amplified using RT-PCR and detected using fluorescent reporter dye probes specific for SARS-CoV-2, Flu and RSV. PRINCIPLE OF THE TEST The detection is done in one step real time RT format where the reverse transcription and the subsequent amplification of specific target sequence occur in the same reaction well. The isolated RNA target is transcribed generating complementary DNA by reverse transcriptase which is followed by the amplification of two conserved regions of N gene (N1 and N2) for SARS-CoV-2, a conserved region of the M1 gene for Flu A/B and a conserved region of the N gene for RSV A/B using specifics primers and a fluorescent-labeled probe. MATERIALS AND REAGENTS ANALYTICAL SENSITIVITY VIASURE SARS-CoV-2, Flu & RSV Real Time PCR Detection Kit has a detection limit (LoD) of 20 genome copies/rxn for SARS-CoV-2, 5 genome copies/rxn for Flu A, 20 genome copies/rxn for Flu B and 10 genome copies/rxn for RSV. CE-IVD marked