SARS-CoV-2 / Influenza Multiplex REAL-TIME PCR Detection Kit

GENERAL INFORMATION Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is the name given to the new coronavirus that originated in 2019 and is the causative agent of COVID-19 (the disease caused by the SARS-CoV-2 virus). The virus was originally known as the 2019-nCoV nomenclature. SARS-CoV-2 is a new coronavirus strain that has not been previously identified in humans. The genome of these viruses consists of single-stranded RNA protected by two protein coatings - nucleocapsid and capsid. Its genetic characteristics are significantly different from SARS-CoV and MERS-CoV. Influenza viruses belong to the Orthomyxoviridaefamily and cause the majority of viral lower respiratory tract infections. Influenza A and B are a significant cause of morbidity and mortality worldwide, considering that elderly and compromised individuals are especially at risk of developing severe illness and complications such as pneumonia. SARS-CoV-2/SARS-CoV Multiplex REAL-TIME PCR Detection Kit is an in vitro diagnostics kit for the qualitative simultaneous detection and differentiation of the RNA of the Influenza A (FluA), Influenza B (FluB) and/or SARS-CoV-2 viruses, starting from purified RNA from human clinical samples of different origin, such as oropharyngeal swabs, bronchoalveolar lavage, endotracheal aspirate, nasopharyngeal aspirate, sputum. PRINCIPLE OF THE TEST The PCR-mix contains four target-specific probes bearing reporter fluorescent dyes (Fam, Hex, Rox and Cy5) and quencher molecules. Once hybridized to a target sequence, the probes become activated and the fluorescence emittion increases proportionally to target sequence amplification. The kit includes the Internal control (RNAIC), which is intended to assess the quality of the RNA extraction and polymerase chain reaction. The application of four fluorescent dyes makes it possible to register the results of different amplification reactions taking place simultaneously in one tube. With this test it is possible to differentiate between Influenza A, Influenza B and SARS-CoV-2 in one sample. MATERIALS AND REAGENTS Paraffin sealed PCR-mix SARS-CoV-2/Influenza RT-PCR-buffer Enzyme Taq/RT Internal control (RNA-IC) Positive control RESULTS INTERPRETATION