ELISA Dynein heavy chain domain-containing protein 1 (DNHD1)

Reactivity: (Homo sapiens) UniProt:Q96M86 Abbreviation:DNHD1 Alternative Names:C11orf47; CCDC35; DHCD1; DKFZp434G0812; DKFZp686J0796; DKFZp686N1238; DNHD1L; FLJ00251; FLJ32752; FLJ35709; FLJ39625; FLJ43897; FLJ46184; MGC133191; DNHD1 variant protein|coiled-coil domain containi Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.112 ng/mL Intra-AssayCV:?5.7% Inter-AssayCV:?8.3% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DNHD1 in samples. An antibody specific for DNHD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNHD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DNHD1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNHD1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DNHD1 Belongs to the dynein heavy chain family. Dynein was first isolated from Tetrahymena cilia four decades ago. The analysis of the primary structure of the dynein heavy chain and the discovery that many organisms express mµLtiple dynein heavy chains have led to two insights. One, dynein, whose motor domain comprises six AAA modµLes and two potential mechanical levers, generates movement by a mechanism that is fundamentally different than that which underlies the motion of myosin and kinesin. And two, organisms with cilia or flagella express approximately 14 different dynein heavy chain genes, each gene encodes a distinct dynein protein isoform, and each isoform appears to be functionally specialized. Sequence comparisons demonstrate that functionally equivalent isoforms of dynein heavy chains are well conserved across species. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).