ELISA Dual specificity protein phosphatase 5 (DUSP5)

Reactivity: (Homo sapiens) UniProt:Q16690 Abbreviation:DUSP5 Alternative Names:DUSP; HVH3; VH1-like phosphatase 3|serine/threonine specific protein phosphatase Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.053 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.1% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DUSP5 in samples. An antibody specific for DUSP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUSP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DUSP5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DUSP5 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regµLate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellµLar proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellµLar localization, and different modes of inducibility of their expression by extracellµLar stimµLi. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).